生物谷报道：随着蛋白质组学的发展，大规模研究蛋白间相互作用已成为重要的研究方向之一。这篇是刚刚出版的PNAS上一篇报道，作用研究了酵母膜表面705种蛋白，以及其中536种蛋白间的至少1985种相互联系和作用。这种研究为今后更广泛的物种的蛋白质间相互作用提供示范。
原文链接：
http://www.pnas.org/cgi/content/full/102/34/12123
Full Text (PDF)
We carried out a large-scale screen to identify interactions between integral membrane proteins of Saccharomyces cerevisiae by using a modified split-ubiquitin technique. Among 705 proteins annotated as integral membrane, we identified 1,985 putative interactions involving 536 proteins. To ascribe confidence levels to the interactions, we used a support vector machine algorithm to classify interactions based on the assay results and protein data derived from the literature. Previously identified and computationally supported interactions were used to train the support vector machine, which identified 131 interactions of highest confidence, 209 of the next highest confidence, 468 of the next highest, and the remaining 1,085 of low confidence. This study provides numerous putative interactions among a class of proteins that have been difficult to analyze on a high-throughput basis by other approaches. The results identify potential previously undescribed components of established biological processes and roles for integral membrane proteins of ascribed functions.
