生物谷报道：国际顶尖植物学权威杂志“The Plant Cell”日前发表了武汉大学生命科学学院博士生陈荣智的研究论文，该项研究成果对于杂交水稻的三系育种具有重要作用。
该论文题为《水稻尿苷二磷酸葡萄糖焦磷酸化酶基因Ugp1对花粉发育过程中的胼胝质沉积是必需的，它的共抑制导致一种新型温敏核不育表型》，运用电喷雾串联质谱鉴定出水稻差异表达蛋白UGPase并克隆了其编码基因Ugp1，随后应用基因超量表达和RNA干扰技术通过水稻转基因对Ugp1基因的功能进行了详细研究，阐明了Ugp1基因在水稻生长发育中的重要功能。同时还发现Ugp1共抑制水稻表现为一种新型的温度敏感细胞核不育类型，并证明Ugp1基因的温度敏感型剪接是温敏核不育Ugp1共抑制水稻育性恢复的分子基础。
陈荣智是武汉大学生命科学学院2002级博士生，师从著名的植物遗传学家何光存教授。
原始出处:
First published online March 30, 2007; 10.1105/tpc.106.044123
The Plant Cell 19:847-861 (2007)
Rice UDP-Glucose Pyrophosphorylase1 Is Essential for Pollen Callose Deposition and Its Cosuppression Results in a New Type of Thermosensitive Genic Male Sterility[W],[OA]
Rongzhi Chen, Xiao Zhao, Zhe Shao, Zhe Wei, Yuanyuan Wang, Lili Zhu, Jie Zhao, Mengxiang Sun, Ruifeng He and Guangcun He1
Key Laboratory of Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China
1 To whom correspondence should be addressed. E-mail gche@whu.edu.cn ; fax 86-27-68752327.
UDP-glucose pyrophosphorylase (UGPase) catalyzes the reversible production of glucose-1-phosphate and UTP to UDP-glucose and pyrophosphate. The rice (Oryza sativa) genome contains two homologous UGPase genes, Ugp1 and Ugp2. We report a functional characterization of rice Ugp1, which is expressed throughout the plant, with highest expression in florets, especially in pollen during anther development. Ugp1 silencing by RNA interference or cosuppression results in male sterility. Expressing a double-stranded RNA interference construct in Ugp1-RI plants resulted in complete suppression of both Ugp1 and Ugp2, together with various pleiotropic developmental abnormalities, suggesting that UGPase plays critical roles in plant growth and development. More importantly, Ugp1-cosuppressing plants contained unprocessed intron-containing primary transcripts derived from transcription of the overexpression construct. These aberrant transcripts undergo temperature-sensitive splicing in florets, leading to a novel thermosensitive genic male sterility. Pollen mother cells (PMCs) of Ugp1-silenced plants appeared normal before meiosis, but during meiosis, normal callose deposition was disrupted. Consequently, the PMCs began to degenerate at the early meiosis stage, eventually resulting in complete pollen collapse. In addition, the degeneration of the tapetum and middle layer was inhibited. These results demonstrate that rice Ugp1 is required for callose deposition during PMC meiosis and bridges the apoplastic unloading pathway and pollen development.
