生物谷报道：新年伊始，国际著名杂志《生物化学》( Journal of Biological Chemistry，283：461-468)发表了由昆明植物所李唯奇研究员与美国科学家合作的论文“Differential degradation of extraplastidic and plastidic lipids during freezing and post-freezing recovery in Arabidopsis Thaliana”。该研究利用基于ESI-MS/MS的脂类组学方法(Lipidomics)，分析比较了拟南芥在响应低温胁迫的冻和冻融过程中约120种膜脂分子的组成变化规律以及磷脂酶D-a1和d在其中的作用。该研究表明，不同类型的膜脂分子或细胞定位不同的膜脂分子，在冻害和冻融过程中发生不同变化；抑制磷脂酶D-a1和d的表达对低温诱导的磷脂水解产生了不同的作用。
据悉，低温胁迫是影响植物的生长发育、地理分布和作物产量的主要环境因素，细胞膜是植物低温伤害产生的主要部位，膜和膜脂分子变化是植物感应温度变化的初始信号之一。植物响应低温胁迫有三个不同阶段，即冷驯(cold-acclimation)、冷冻(freezing)和冻融(post-freezing recovery)。
该研究利用组学方法，详细和有规模的解析了两种生态型和四种基因型的拟南芥膜脂分子在三个低温胁迫阶段中的变化，为进一步揭示植物响应低温胁迫的机制提供了重要的基础。该项研究已被《自然中国》(Nature China)杂志选为来自中国大陆和香港的突出科学研究成果，论文的研究亮点已经刊登在该杂志的网站上http://www.nature.com/nchina/featured/112007.html
生物谷推荐英文原文：
J. Biol. Chem., Vol. 283, Issue 1, 461-468, January 4, 2008
Differential Degradation of Extraplastidic and Plastidic Lipids during Freezing and Post-freezing Recovery in Arabidopsis thaliana*
Weiqi Li1, Ruiping Wang, Maoyin Li, Lixia Li, Chuanming Wang¶, Ruth Welti||, and Xuemin Wang
From the Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming 650204, China, the Department of Biology, University of Missouri and Donald Danforth Plant Science Center, St. Louis, Missouri 63121, the ¶Department of Biology, Honghe University, Mengzi, Yunnan 661100, China, and the ||Kansas Lipidomics Research Center, Division of Biology, Kansas State University, Manhattan, Kansas 66506
Changes in membrane lipid composition play important roles in plant adaptation to and survival after freezing. Plant response to cold and freezing involves three distinct phases: cold acclimation, freezing, and post-freezing recovery. Considerable progress has been made toward understanding lipid changes during cold acclimation and freezing, but little is known about lipid alteration during post-freezing recovery. We previously showed that phospholipase D (PLD) is involved in lipid hydrolysis and Arabidopsis thaliana freezing tolerance. This study was undertaken to determine how lipid species change during post-freezing recovery and to determine the effect of two PLDs, PLD1 and PLD, on lipid changes during post-freezing recovery. During post-freezing recovery, hydrolysis of plastidic lipids, monogalactosyldiacylglycerol and plastidic phosphatidylglycerol, is the most prominent change. In contrast, during freezing, hydrolysis of extraplastidic phospholipids, phosphatidylcholine and phosphatidylethanolamine, occurs. Suppression of PLD1 decreased phospholipid hydrolysis and phosphatidic acid production in both the freezing and post-freezing phases, whereas ablation of PLD increased lipid hydrolysis and phosphatidic acid production during post-freezing recovery. Thus, distinctly different changes in lipid hydrolysis occur in freezing and post-freezing recovery. The presence of PLD1 correlates with phospholipid hydrolysis in both freezing and post-freezing phases, whereas the presence of PLD correlates with reduced lipid hydrolysis during post-freezing recovery. These data suggest a negative role for PLD1 and a positive role for PLD in freezing tolerance.
Received for publication, August 13, 2007 , and in revised form, October 24, 2007.
* The work was supported by grants from the United States Department of Agriculture, the Kansas State University (KSU) Plant Biotechnology Center, the National Basic Research Program of China (Grant 2006CB100100), Knowledge Innovation Program of CAS (KSCX2-YW-N-014), NSFC (30670474), NSF (MCB 0455318, IOS 0454866, DBI 0521587, and Kansas NSF EPSCoR award, EPS-0236913), with support from the State of Kansas through the Kansas Technology Enterprise Corporation and KSU, as well from United States Public Health Services Grant P20 RR016475 from the INBRE program of the National Center for Research Resources. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 To whom correspondence should be addressed. Tel.: 86-871-522-3025; Fax: 86-871-522-3018; E-mail: weiqili@mail.kib.ac.cn .
