对30个候选基因所做的一个RNA干涉筛选，识别出Lin28（let-7 microRNA处理的一个负调控因子）为原始生殖细胞发育（这是发育中的胚胎内所发生的一个过程，它选择注定要产生精子和卵子的细胞）的一个潜在关键调控因子。另外，Lin28水平在原发性人类生殖细胞肿瘤中升高，说明它也许也与生殖细胞恶性肿瘤有关。（生物谷Bioon.com）
生物谷推荐原始出处：
Nature 460, 909-913 (13 August 2009) | doi:10.1038/nature08210
A role for Lin28 in primordial germ-cell development and germ-cell malignancy
Jason A. West1,2, Srinivas R. Viswanathan1,2, Akiko Yabuuchi1,2, Kerianne Cunniff1,2, Ayumu Takeuchi1,2, In-Hyun Park1,2, Julia E. Sero3, Hao Zhu1,2, Antonio Perez-Atayde3, A. Lindsay Frazier1,4, M. Azim Surani5 & George Q. Daley1,2,6,7
1 Division of Pediatric Hematology/Oncology, Children's Hospital Boston and the Dana-Farber Cancer Institute,
2 Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard Stem Cell Institute, Boston, Massachusetts 02115, USA
3 Department of Pathology, Children's Hospital Boston and Harvard Medical School, Boston, Massachusetts 02115, USA
4 Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
5 Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
6 Manton Center for Orphan Disease Research, Boston, Massachusetts 02115, USA
7 Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA
The rarity and inaccessibility of the earliest primordial germ cells (PGCs) in the mouse embryo thwart efforts to investigate molecular mechanisms of germ-cell specification. stella (also called Dppa3) marks the rare founder population of the germ lineage1, 2. Here we differentiate mouse embryonic stem cells carrying a stella transgenic reporter into putative PGCs in vitro. The Stella+ cells possess a transcriptional profile similar to embryo-derived PGCs, and like their counterparts in vivo, lose imprints in a time-dependent manner. Using inhibitory RNAs to screen candidate genes for effects on the development of Stella+ cells in vitro, we discovered that Lin28, a negative regulator of let-7 microRNA processing3, 4, 5, 6, is essential for proper PGC development. Furthermore, we show that Blimp1 (also called Prdm1), a let-7 target and a master regulator of PGC specification7, 8, 9, can rescue the effect of Lin28 deficiency during PGC development, thereby establishing a mechanism of action for Lin28 during PGC specification. Overexpression of Lin28 promotes formation of Stella+ cells in vitro and PGCs in chimaeric embryos, and is associated with human germ-cell tumours. The differentiation of putative PGCs from embryonic stem cells in vitro recapitulates the early stages of gamete development in vivo, and provides an accessible system for discovering novel genes involved in germ-cell development and malignancy.
