成年鱼类大脑有大量放射状胶质细胞，直至成年期，但放射状胶质细胞在成年动物神经发生过程中的作用尚不明了。课题组的前期研究表明，樱花钩吻鲑与其他鱼种不同的是，酪氨酸羟化酶免疫定位第三脑室和大脑尾区的长放射状突起的细胞中。发表在2013年1月第1期《中国神经再生研究（英文版）》杂志的“Features of adult neurogenesis and neurochemical signaling in the Cherry salmon Oncorhynchus masou brain”的研究表明，在樱花钩吻鲑生后不同发育阶段，室周细胞表现出神经元和胶质细胞，包括放射状胶质细胞的形态，并包含多种神经化学物质。大脑脚背盖背内侧、髓质和脊髓中央灰质层、间脑室周围区的细胞增殖区出现酪氨酸羟化酶、γ氨基丁酸和NADPH黄递酶免疫阳性细胞。Pax6免疫定位检测揭示鲑鱼在生后不同发育阶段脑结构变化，这些变化也得到酪氨酸羟化酶和γ氨基丁酸免疫组化证实。(生物谷Bioon.com)
doi:10.3969/j.issn.1673-5374.2013.01.002
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Features of adult neurogenesis and neurochemical signaling in the Cherry salmon Oncorhynchus masou brain
Evgeniya V. Pushchina1, Dmitry K. Оbukhov2, Anatoly A. Varaksin1
We investigated the distribution of gamma aminobutyric acid, tyrosine hydroxylase and nitric oxide-producing elements in a cherry salmon Oncorhynchus masou brain at various stages of postnatal ontogenesis by immunohistochemical staining and histochemical staining. The periventricular region cells exhibited the morphology of neurons and glia including radial glia-like cells and contained several neurochemical substances. Heterogeneous populations of tyrosine hydroxylase-, gamma aminobutyric acid-immunoreactive, аs well as nicotinamide adenine dinucleotide phosphate diaphorase-positive cells were observed in proliferating cell nuclear antigen-immunoreactive proliferative zones in periventricular area of diencephalon, central grey layer of dorsomedial tegmentum, medulla and spinal cord. Immunolocalization of Pax6 in the cherry salmon brain revealed a neuromeric construction of the brain at various stages of postnatal ontogenesis, and this was confirmed by tyrosine hydroxylase and gamma aminobutyric acid labeling.
