生物谷报道：有研究表明，甲状腺可以诱发自身免疫反应，因为胸腺是免疫系统中的T细胞的发育场所，如果T细胞不能把机体中的成千上万种蛋白识别为自身蛋白，那么就可能由胸腺诱发自身免疫反应。旧金山的加利福尼亚大学(University of California, San Francisco，UCSF)的研究人员最近证实了这个新出现的观点，即自身免疫反应可能起源于这个免疫系统的发源地——胸腺，而不仅是发生在自身免疫性疾病发生的场所，例如1型糖尿病可能不仅发生在胰腺，风湿性关节炎可能不仅发生在关节，（都有可能发生在胸腺）。结果发表在11月20日的经验医学杂志的网络版上，并可能会发表在该杂志的11月27日的印刷版上。
这个研究结果来自人类自身免疫性疾病的大鼠模型，同时这个研究结果显示能够有效治疗自身免疫性疾病的方法应该不仅仅针对发生自身免疫性疾病的外周组织，而且应该同时包括产生T细胞和自身蛋白或是首先发生反应产生自身抗体的组织——胸腺。
当T细胞在胸腺内遭遇机体全部的蛋白质组分的时候，那些带有可以识别“自身”蛋白或是抗原的T细胞便被清除（灭活），避免以后发生攻击机体的自身免疫反应。这项新的研究显示，哪怕是只有一种自身抗原没有被识别为“自身”成分并加以清除，这个识别缺欠就有可能会导致视网膜发生严重的自身免疫性疾病。该清除过程主要涉及细胞核内的一种被称为Aire的蛋白（它是自身免疫的调节者），它可以调控胸腺内大约300到1,000种抗原的表达。缺乏正常Aire基因表达的人类和大鼠易患包括针对甲状腺、肾上腺、卵巢和眼睛的多种自身免疫性疾病。
英文原文：
Autoimmune disease triggered if T cells miss a single protein early on
Scientists have discovered that autoimmunity can be triggered in the thymus, where the immune system's T cells develop, if T cells fail to recognize just one of the body's thousands of proteins as "self." The research confirms an emerging view that autoimmunity can start in this cradle of the immune system, and not only at the sites where autoimmune diseases emerge, such as the pancreas in the case of type 1 diabetes, or the joints in rheumatoid arthritis.
The discovery, from a mouse model of a human autoimmune condition, suggests that effective strategies to treat autoimmune disease should target not only the "peripheral" sites where autoimmune disease is active, but also the thymus -- the organ where T cells and self-proteins, or self-antigens, first interact.
The research was led by investigators at the University of California, San Francisco (UCSF). It was published online November 20 by the Journal of Experimental Medicine and will appear in the journal's print edition November 27.
T cell soldiers encounter the body's full array of proteins in the thymus, and those T cells with receptors that recognize "self" proteins, or antigens, normally are purged to avoid autoimmune attacks in the body later on. The new research showed that if just one of the body's antigens is not recognized as "self," this single failure can lead to a severe autoimmune disease in the retina.
"The thymus is like a filter," said Mark Anderson, MD, PhD, assistant professor of medicine at the UCSF Diabetes Center, and senior author of a scientific paper describing the discovery. "It is removing or pulling out autoreactive T cells. What this new study shows is if just one self-antigen is missing as the T cells go through the filter, it looks like this alone can lead to an autoimmune disease."
"The finding supports the promise of treatments targeting individual body proteins or antigens since we have shown that a single self-antigen can trigger disease," he added.
A similar mechanism may be at play involving other autoimmune diseases such as type 1 diabetes, Anderson said. Immunologists have demonstrated that insulin is expressed in the thymus ?not just in the pancreas. Studies have shown that people who are protected from diabetes express high levels of insulin in the thymus, while those who are predisposed express lower levels of insulin in this organ.
"What we think is that 'more is better' in the thymus," Anderson says. "If you have more insulin in the thymus, then there is a better chance that potentially destructive insulin-specific T cells will encounter insulin as self and be filtered out."
In the thymus, immature T cells display on their surface many thousands of unique receptors, generated by random gene rearrangements. This strategy allows the receptors to recognize the tremendous diversity of invading pathogens. In the process, however, they also develop receptors that bind to the body's own proteins. These T cells are normally eliminated, avoiding the plague of autoimmunity.
A clue to how the elimination process is controlled came from previous work involving a protein in the cell nucleus called Aire (for autoimmune regulator), which regulates the expression of some 300 to 1,000 antigens in the thymus. Humans and mice lacking the normal Aire gene suffer from multiple autoimmune diseases including diseases that target the thyroid, adrenal, ovary, and eye.
In 2002, Anderson, then at Harvard Medical School, and colleagues there demonstrated that knocking out the Aire gene in the mouse thymus led to failures of expression of a number of genes in peripheral tissues, resulting in autoimmune diseases in those tissues -- the first direct evidence linking gene knockouts in the thymus to autoimmune defects in body tissues. The study, however, did not link a specific organ autoimmune attack with a specific protein missing in the thymus.
In the new study, the researchers carried out a detailed analysis of the autoimmune attack that is directed against the eye in Aire-deficient mice. What the team found was that the immune system was mainly targeting a single eye protein called IRBP despite the fact that several eye-specific proteins were missing in the thymus of Aire knockout mice. The team then went on to show that IRBP was expressed in the thymus under the control of Aire and that knockout mice lacking the IRBP protein were protected from the disease because they don't express the protein that the immune system is targeting.
In a key, final part of the new study, Anderson and his colleagues showed that if mice without a thymus gland ?so-called "nude" mice ?received a normal thymus lacking only IRBP, they developed the autoimmune eye disease. The autoimmune attack occurred even though the mice had normally functioning IRBP in their retinas. The final finding demonstrated that failure of T cells in the thymus to recognize IRBP as a self-protein was sufficient to cause the autoimmune disorder in the retina.
The scientists hope that better understanding of interactions in the thymus can lead to earlier, more effective treatment of autoimmune diseases.
"When we see autoimmune disease in the clinic, we are usually looking at it in a relatively late stage. Tissue is already damaged, antigen expression is ramped up and the immune response is spreading to other self-antigens," Anderson said. "If we can also train our focus on the thymus, where we know at least some of the autoimmune disease is triggered, we may be able to determine just what self-antigens are important and shut down the autoimmune process targeting those self -antigens."
The team is collaborating with Jeffrey Bluestone, PhD, director of the UCSF Diabetes Center, in preclinical studies to see if T cell autoimmune attacks on IRBP can be modulated to prevent the autoimmune eye disease.
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Lead author is Jason DeVoss, PhD, a postdoctoral scientist in Anderson's laboratory.
Co-authors include Lawrence Fong, MD, PhD, UCSF assistant professor of hematology and oncology; Yafei Hou, PhD, a postdoc in Fong's lab; Wen Lu, BS, and Kellsey Johannes, BA, both research assistants in Anderson's lab.
Also: Gregory Liou, PhD, associate professor of ophthalmology at the Medical College of Georgia; Howard Chang, MD, PhD, assistant professor dermatology at Stanford University School of Medicine; John Rinn, PhD, a postdoctoral scientist in Chang's lab; and Rachel Caspi, PhD, section head, Laboratory of Immunology, National Eye Institute.
The research is supported in part by the National Institutes of Health.
UCSF is a leading university that advances health worldwide by conducting advanced biomedical research, educating graduate students in the life sciences and health professions, and providing complex patient care.
原文链接：http://www.eurekalert.org/pub_releases/2006-11/uoc--adt112106.php
