在tetramer-hybridization原位内SIV免疫效应细胞和在一受感染猴子的子宫颈内一感染细胞的特写。
图片来源: Qingsheng Li, Ashley Haase and Tim Leonard
据3月27日的《科学》杂志报道说，Qingsheng Li及其同僚说，一种用肉眼观察免疫系统对某种病毒感染的早期反应的新方法可以帮助人们预测感染的病毒是否会得到遏制、被消灭，或会进行活跃的繁殖。 这些发现可能被证明对测试HIV-1 疫苗及对其它的病毒感染的早期控制是有用的。
研究人员说，病毒感染的后果“在某种意义上来说是一种数字游戏”，即这是在病毒繁殖的速度和规模与免疫系统反应的速度和规模之间进行的一种竞赛。 Li及其同僚结合了在动物感染模型中的2种技术来观看免疫系统对猴免疫缺陷病毒（SIV）以及淋巴细胞性脉络丛脑膜炎病毒的某些病毒株所产生的免疫反应。 他们显示，以病毒为目标的T细胞的爆发规模和时机可以帮助人们预测免疫反应是否会遏制和消除该种病毒的感染。（生物谷Bioon.com）
生物谷推荐原始出处：
Science 27 March 2009:DOI: 10.1126/science.1168676
Visualizing Antigen-Specific and Infected Cells in Situ Predicts Outcomes in Early Viral Infection
Qingsheng Li,1 Pamela J. Skinner,2 Sang-Jun Ha,3 Lijie Duan,1 Teresa L. Mattila,2 Aaron Hage,2 Cara White,2 Daniel L. Barber,4 Leigh O'Mara,3 Peter J. Southern,1 Cavan S. Reilly,5 John V. Carlis,6 Christopher J. Miller,7 Rafi Ahmed,3 Ashley T. Haase1*
In the early stages of viral infection, outcomes depend on a race between expansion of infection and the immune response generated to contain it. We combined in situ tetramer staining with in situ hybridization to visualize, map, and quantify relationships between immune effector cells and their targets in tissues. In simian immunodeficiency virus infections in macaques and lymphocytic choriomeningitis virus infections in mice, the magnitude and timing of the establishment of an excess of effector cells versus targets were found to correlate with the extent of control and the infection outcome (i.e., control and clearance versus partial or poor control and persistent infection). This method highlights the importance of the location, timing, and magnitude of the immune response needed for a vaccine to be effective against agents of persistent infection, such as HIV-1.
1 Department of Microbiology, Medical School, University of Minnesota, Minneapolis, MN 55455, USA.
2 Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, MN55108, USA.
3 Emory Vaccine Center, Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA.
4 Immunobiology Section, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA.
5 Division of Biostatistics, School of Public Health, University of Minnesota, Minneapolis, MN 55455, USA.
6 Department of Computer Science and Engineering, Institute of Technology, University of Minnesota, Minneapolis, MN 55455, USA.
7 Center for Comparative Medicine, California National Primate Research Center, University of California, Davis, CA 95616, USA.
