中国神经再生研究（英文版）》杂志于2012年7月19期出版的一项关于“Immunotherapy of rat glioma without accumulation of CD4+CD25+FOXP3+ regulatory T cells”的研究显示，应用全胶质瘤细胞裂解物致敏树突状细胞，联合过继性T细胞建立了一种新的免疫方法。
接种后21d，联合免疫的载瘤鼠脑肿瘤体积减小，肿瘤内特异性细胞毒性T淋巴细胞的细胞毒性反应强烈，外周血CD4+ CD25 + FOXP3+调节性T细胞比例明显下降，存活时间延长。
作者认为全胶质瘤细胞裂解物致敏树突状细胞联合过继性T细胞可有效地抑制大鼠脑胶质瘤的生长，提高抗肿瘤能力，也避免了CD4+ CD25 + FOXP3+调节性T细胞的积累，可为临床治疗恶性脑胶质瘤提供强有力的免疫治疗方案和科学依据。（生物谷Bioon.com）
doi:10.3969/j.issn.1673-5374
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Immunotherapy of rat glioma without accumulation of CD4+ CD25+ FOXP3+ regulatory T cells
Enshan Feng1 , Haili Gao2 , Wei Su2 , Chunjiang Yu1
Immunotherapy may be used for the treatment of glioblastoma multiforme; however, the induced immune response is inadequate when either T cells or dendritic cells are used alone. In this study we established a novel vaccine procedure in rats, using dendritic cells pulsed with C6 tumor cell lysates in combination with adoptive transfer of T lymphocytes from syngenic donors. On day 21 after tumor inoculation, all the rats were sacrificed, the brains were harvested for calculation of glioma volume, cytolytic T lymphocyte responses were measured by cytotoxic assay, and the frequency of regulatory T lymphocytes (CD4+ CD25+ FOXP3+ ) in the peripheral blood was inves- tigated by flow cytometric analysis. The survival rate of rats bearing C6 glioma was observed. Results showed that the co-immunization strategy had significant anti-tumor potential against the pre-established C6 glioma, and induced a strong cytolytic T lymphocyte response in rats. The frequency of peripheral blood CD4+ CD25+ FOXP3+ regulatory T lymphocytes was significantly decreased following the combination therapy, and the rats survived for a longer period. Experi- mental findings indicate that the combined immunotherapy of glioma cell lysate-pulsed dendritic cell vaccination following adoptive transfer of T cells can effectively inhibit the growth of gliomas in rats, boost anti-tumor immunity and produce a sustained immune response while avoiding the accumulation of CD4+ CD25+ FOXP3+ regulatory T lymphocytes.
