Mycosystema
菌 物 学 报 15 March 2008, 27(2): 224-229
jwxt@im.ac.cn
ISSN1672-6472 CN11-5180Q
2008 Institute of Microbiology, CAS, all rights reserved.
左登平 李春如 黄勃 何亚琼 樊美珍 李增智
安徽农业大学微生物防治省重点实验室 合肥 230036
摘 要：采用PCR技术，以rDNA-ITS区为分子指标，对台湾虫草Cordyceps formosana及其无性型黄山被毛孢Hirsutella huangshanensis进行测序和比较分析，以进一步确定两者的对应关系。结果表明：两者的序列完全相同，从而在分子水平上证明了黄山被毛孢是台湾虫草的无性阶段。系统进化树显示台湾虫草的无性型归为被毛孢属较为合理。
关键词：黄山被毛孢，rDNA-ITS 区，虫生真菌
Molecular identification for connection of Cordyceps formosana and its Hirsutella anamorph
ZUO Deng-Ping LI Chun-Ru* HUANG Bo HE Ya-Qiong FAN Mei-Zhen LI Zeng-Zhi
Anhui Provincial Key Laboratory for Microbial Pest Control, Anhui Agricultural University, Hefei 230036, China
Abstract: The genes of the 5.8S rRNA and the complete internal transcribed spacer (ITS) regions from Cordyceps formosana and Hirsutella huangshanensis were sequenced and compared. The results showed that Cordyceps formosana and Hirsutella huangshanensis had the same ITS1-5.8S-ITS2 nucleotide sequences, strongly supporting the fact that Cordyceps formosana was the teleomorph of Hirsutella huangshanensis. Molecular phylogenetic analysis demonstrated that the conidial state of C. formosana belonged to the genus Hirsutella.
Key words: Hirsutela huangshanensis, rDNA-ITS, entomopathogenic fungi
