微生物学通报 NOV 20, 2008, 35(11): 1760~1763
Microbiology 2008 by Institute of Microbiology, CAS
一株家养野猪源猪圆环病毒2型的分离与鉴定
王海燕 高 崧* 刘秀梵
(扬州大学 农业部畜禽传染病学重点开放实验室 扬州 225009)
摘 要: 应用PCR方法从临诊疑似断奶仔猪多系统衰竭综合征(PMWS)家养野猪病例的淋巴结和脾脏中扩增出预期长度的猪圆环病毒2型(PCV2)的DNA片段, 在Dulac细胞中进行分离和培养, 扩增全基因组序列后进行同源性分析。结果显示, 扩增产物与家猪源参考毒株的序列同源性均在98%以上, 该病毒与家猪源病毒差异不大。（生物谷Bioon.com）
关键词: 家养野猪, 猪圆环病毒2 型, 分离, 鉴定
Isolation and Identification of Porcine Circovirus Type 2 Virus from a Domesticated Wild Boar
WANG Hai-Yan GAO Song* LIU Xiu-Fan
(Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture, Yangzhou University, Yangzhou 225009)
Abstract: The nucleic acid fragment of porcine circovirus type 2(PCV2)was amplified using PCR from the tissues of a domesticated wild boar with suspected postweaning multisystemic wasting syndrome (PMWS). The sample were then inoculated into the Dulac cells and passaged for 8 generation. The 4 nucleic acid fragments covered the complete genome for PCV2 were obtained by over-lapping PCR and sent to sequence. The sequence of genome was compared with other 9 strains originated from piglets in the same area. The result showed that these strains were in high homology.
Keywords: Domesticated wild boar, Porcine circovirus type 2, Isolation, Identification
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