菌物学报 15 July 2009, 28(4):496～503
陇南地区不同海拔区域内小麦条锈菌群体遗传多样性的分析
陆宁海1,2 王建锋1,2 詹刚明1 黄丽丽1 康振生1,2*
1西北农林科技大学植物保护学院 杨凌 712100
2西北农林科技大学 陕西省农业分子生物学重点实验室 杨凌 712100
摘 要：小麦条锈病是世界范围内小麦上最重要的流行性病害之一，可造成严重的产量损失。陇南地区是我国小麦条锈菌主要越夏易变区和新小种发源地，了解该地区不同海拔高度区域内条锈菌遗传多样性有重要意义。本研究采用TP-M13-SSR 荧光标记技术对11个种群330个小麦条锈菌分离株基因组DNA进行了SSR标记分析。不同海拔区域的条锈菌遗传多样性有明显的差异，高山区的遗传多样性比较丰富，半山区次之，川道区相对比较低。不同生态区域内，小麦条锈菌群体遗传分化程度不同，高山区和半山区遗传分化程度大，基因流小，川道区群体遗传分化程度比较小，基因流大。来自不同海拔区域的菌系具有相同的基因型， 这一结果从分子水平证明了在陇南地区小麦条锈菌在山区与川地之间存在广泛的菌源交流, 可就地完成周年循环。
关键词：小麦条锈菌，遗传分化，海拔，SSR标记
SSR analysis of population genetic diversity of Puccinia striifornis f. sp. tritici in different altitude regions of Longnan, Gansu
LU Ning-Hai1, 2 WANG Jian-Feng1, 2 ZHAN Gang-Ming1 HUANG Li-Li1 KANG Zhen-Sheng1, 2*
1College of Plant Protection, Northwest Agriculture & Forestry University, Yangling 712100, China
2Shaanxi Key Laboratory of Molecular Biology, Northwest Agriculture & Forestry University, Yangling 712100, China
Abstract: Stripe rust (or yellow rust) of wheat (Triticum aestivum), caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat worldwide. It is very important to research the population genetic diversity of P. striifornis f. sp. tritici in different altitude regions of Longnan, one of the largest and the most important over-summering areas in China. Using the TP-M13-SSR, population genetic diversity and variation of P. striiformis f. sp. tritici, containing 330 isolates 11 collections from different altitude area of Longnan, were investigated. The important conclusion is that P. striifornis f. sp. tritici populations from different altitude areas were genetically different. Genetic diversity for the pathogen population from high mountainous area is much higher than that from mountainside area or lowlands. The genetic differentiation for the populations obtained from high mountain and mountainside areas was higher than that of the population from the lowlands, suggesting the limited gene flow in mountain area, and extensive gene exchange in lowlands. Our molecular data confirm that the stripe rust pathogen is able to exchange between lowland areas and high mountain areas in the region.
Key words: stripe rust of wheat, genetic differentiation, SSR marker, altitude
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