微生物学通报 MAY 20,2010,37(5):637～644
一株耐低温纤维素酶高产菌株的筛选、鉴定和产酶的初步试验
黄玉兰 李征 刘晓宁 王培培 张杰*
(四川大学生命科学学院资源微生物与微生物技术四川省重点实验室 四川 成都 610064)
摘 要: 从若尔盖高寒湿地距表层80 cm处土壤中筛选出一株纤维素酶高产菌株XW-1。根据形态学、生理生化特征以及16S rDNA核酸序列分析结果表明, 该菌属于缺陷短波单胞菌(Brevundimonas sp.)。对该菌产酶条件研究表明, XW-1在含0.5% CMC-Na条件下, 20°C培养3 d后出现最高酶活, 达到15.6 U/mL。对其酶学性质初步研究表明, 该菌株所产纤维素酶的最适pH为6.0, 最适反应温度为20°C, 15°C时相对酶活达到80%, 并且在5°C时, 相对酶活仍能保持56%。
关键词: 若尔盖高寒湿地, 低温纤维素酶, 短波单胞菌, 酶学性质
Screening and Identification of a Cold-adapted Cellulase-producing Bacteria
HUANG Yu-Lan LI Zheng LIU Xiao-Ning WANG Pei-Pei ZHANG Jie*
(Key Laboratory of Microbial-resources and Microbial-technology, College of Life Sciences,
Sichuan University, Chengdu, Sichuan 610064, China)
Abstract: Strain XW-1 producing extracellular cellulose was isolated from 80 cm depth under the sur-face layer at Zoige Plateau Alpine Wetland. It was identified as a Brevundimonas sp. by its morpho-logical, the physiological and biochemical properties and 16S rDNA sequence analysis. The results of cellulose producing research showed that the optimum carbon sourse was 0.5% CMC-Na, the optimum temperature was 20°C. Under this condition, the highest cellulose activity reached to 15.6 U/mL in the third day. Enzymatic properties showed that the optimum pH value is 6.0 and the optimum reaction temperature is 20°C, the relative enzyme activity reach to 80% at 15°C, moreover, at 5°C the relative enzyme activity still reach to 56%.
Keywords: Zoige Alpine Wetland, Low cellulose, Brevundimonas, Enzymatic properties
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