投喂低聚木糖对草鱼肠道菌群的影响
熊娟 吴志新 张朋 曲艺 付思思 刘佳佳 陈孝煊*
(华中农业大学水产学院 湖北 武汉 430070)
摘 要: 投喂添加0.1%、0.2%、0.4%和0.6%低聚木糖的基础饲料56 d, 对草鱼(Ctenopharyngodon idellus)肠道菌群进行了研究。分别在投喂前(0 d)和投喂后的第14、28、42和56天取样, 对草鱼肠道大肠杆菌(E. coli)、气单胞菌(Aeromonas)和双歧杆菌(Bifidobacterium)数量进行了分析。结果表明: 基础饲料中添加不同浓度的低聚木糖对草鱼肠道菌群有一定影响, 大肠杆菌数量在28 d达最低值, 其中0.4%组减少的幅度最大, 与对照组比较显著减少(P<0.05); 气单胞菌数量均有减少但与对照组比较差异并不显著; 双歧杆菌数量均有增加, 其中0.4%组在第14天时差异显著(P<0.05)。因此, 饲料中添加0.4%低聚木糖效果最佳, 有利于草鱼肠道菌群保持健康的状态。
关键词: 低聚木糖, 草鱼, 肠道, 菌群
生物谷推荐英文摘要：
Effects of dietary xylo-oligosaccharide on intestinal microflora of grass carp (Ctenopharyngodon idellus)
XIONG Juan WU Zhi-Xin ZHANG Peng QU Yi FU Si-Si LIU Jia-Jia
CHEN Xiao-Xuan*
(College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China)
Abstract: Intestinal microflora of grass carp (Ctenopharyngodon idellus) feeding with xylo-oligosac-charide for 56 days by concentrations of 0.1%, 0.2%, 0.4% and 0.6% respectively was investigated in this paper. Numbers of E. coli, Aeromonas and Bifidobacterium were analyzed before feeding and after 14, 28, 42, 56 days. The results showed that there was a certain influence on the intestinal microflora of grass carp after fed with different concentrations of xylo-oligosaccharides. The quantity of E. coli in the intestine of grass carps was smallest on the 28th day, and compared with the control group, 0.4% group significantly different (P<0.05) has the highest decreasing magnitudes. The quantity of Aeromonas re-duced but there was no significant difference with control. The quantity of Bifidobacterium increased and there was significant difference (P<0.05) between control and 0.4% group on 14th day. Therefore, feedstuffs with xylo-oligosaccharide, which were conducive to maintain a healthy state of intestinal microflora, group with 0.4% has the best effect.
Keywords: Xylo-oligosaccharide, Grass carp, Intestine, Microflora
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