11月28日，国际权威杂志《环境微生物学》Environment Microbiology刊登了浙江大学生命科学学院微生物研究所冯明光教授实验室的两项科研成果；第一项研究成果是冯明光教授发表了题为“Primary roles of two dehydrogenases in the mannitol metabolism and multi-stress tolerance of entomopathogenic fungus Beauveria bassiana”的研究论文，在文中冯明光教授实验室采用基因敲除与回补的方法，成功解析了球孢白僵菌的两种脱氢酶的生物学功能，发现这两种酶在该菌的甘露醇代谢与多胁迫抗逆反应中起着关键作用。
另一成果是冯明光教授发表了题为“A Group III histidine kinase (mhk1) upstream of high-osmolarity glycerol pathway regulates sporulation, multi-stress tolerance and virulence of Metarhizium robertsii, a fungal entomopathogen"的研究论文，冯明光教授实验室利用相同方法（基因敲除与回补），成功解析了在罗伯茨绿僵菌高渗甘油代谢途径上游的组氨酸激酶是调节该菌生长发育、多胁迫抗性和毒力等重要性状的关键激酶，并证明它控制着其下游一系列蛋白激酶和表型相关性状的基因表达。
这两项研究成果均发表在《环境微生物学》Environmental Microbiology杂志上，该杂志影响因子为5.638（IF 5.638 2010）。（生物谷Bioon.com）
doi:10.1111/j.1462-2920.2011.02654.x
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Primary roles of two dehydrogenases in the mannitol metabolism and multi-stress tolerance of entomopathogenic fungus Beauveria bassiana.
Wang ZL, Lu JD, Feng MG
Knockout and complement mutants of mannitol-1-phosphate dehydrogenase (MPD) and mannitol dehydrogenase (MTD) were constructed to probe the roles of both enzymes in the mannitol metabolism and multi-stress tolerances of entomopathogenic fungus Beauveria bassiana. Compared with wild-type and complement mutants, ΔBbMPD lost 99.5% MPD activity for reducing fructose-6-phosphate to mannitol-1-phosphate while ΔBbMTD lost 78.9% MTD activity for oxidizing mannitol to fructose. Consequently, mannitol contents in mycelia and conidia decreased 68% and 83% for ΔBbMPD, and 16% and 38% for ΔBbMTD, accompanied by greatly enhanced trehalose accumulations due to 81-87% decrease in their neutral trehalase expression. Mannitol as mere carbon source in a nitrate-based minimal medium suppressed the colony growth of ΔBbMTD instead of ΔBbMPD, and delayed more conidial germination of ΔBbMTD than ΔBbMPD. Based on median lethal responses, conidial tolerances to H(2) O(2) oxidation, UV-B irradiation and heat stress at 45°C decreased 38%, 39% and 22% in ΔBbMPD, and 18%, 16% and 11% in ΔBbMTD respectively. Moreover, ΔBbMPD and ΔBbMTD lost 14% and 7% of their virulence against Spodoptera litura larvae respectively. Our findings highlight the primary roles of MPD and MTD in mannitol metabolism and their significant contributions to multi-stress tolerances and virulence influential on the biocontrol potential of B. bassiana
