近日，中国科学院植物研究所研究员田世平带领的研究小组，利用双向凝胶电泳结合MALDI-TOF/TOF质谱分析技术，发现灰霉病菌在不同环境pH值条件下胞外分泌组有显著差异，共鉴定出47个差异蛋白点，对应21个不同的胞外蛋白。相关研究成果近期在线发表在国际著名学术期刊Journal of Proteome Research上。
同时，研究发现在pH4条件下，灰霉病菌倾向于分泌更多的蛋白酶类，而在pH6条件下与细胞壁降解相关的酶类被诱导表达。实时定量PCR的分析结果表明，绝大部分差异蛋白的表达调控发生在转录水平上。
灰霉病菌能引起世界上200余种植物发生病害，每年造成巨大的经济损失。该病菌不仅寄主范围广，而且能侵染寄主植物的果实、花器、茎秆、叶片等不同组织。研究灰霉病菌的致病机制，对预防和控制灰霉病的发生具有重要意义。
上述研究结果表明，灰霉病菌可以通过调节分泌蛋白表达谱来响应环境pH值（包括寄主组织pH值）的变化，这为揭示灰霉病菌在不同寄主和组织上复杂的侵染机制提供了重要依据。（生物谷Bioon.com）
doi:10.1021/pr300365f
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Exploring Pathogenic Mechanisms of Botrytis cinerea Secretome under Different Ambient pH Based on Comparative Proteomic Analysis
Boqiang Li,Weihao Wang,Yuanyuan Zong,Guozheng Qin,Shiping Tian,
Botrytis cinerea causes gray mold rot on over 200 plant species worldwide, resulting in great economic loss every year. Cooperation of proteins secreted by B. cinerea plays an important role in its successful infection to host plants. The ambient pH, as one of the most important environmental parameters, can regulate expression of secreted proteins in various fungal pathogens. In the present study, we mainly investigated the effect of ambient pH on secretome of B. cinerea strain B05.10 with a comparative proteomic method based on 2-DE. Distinct differences in secretome of B. cinerea were found between pH 4 and 6 treatments, and 47 differential spots, corresponding to 21 unique proteins, were identified using MALDI-TOF/TOF. At pH 4, more proteins related to proteolysis were induced, whereas most of up-accumulated proteins were cell wall degrading enzymes at pH 6. Analysis of gene expression using quantitative real-time PCR suggests that production of most of these proteins was regulated at the level of tra...
