（图片来源：Lothar Schermelleh and Peter Carlton）
生物谷报道：科学家发明一种新型的显微镜能够展示高清晰度、多色彩的三维画面，它比以前用的传统显微镜能揭示出更多的细节。这种技术叫做三维结构照明（3D-SIM）的新成像技术，并用它在大约100纳米的分辨率下来探索哺乳动物的细胞核。
最好的传统显微镜的分辨率可以放大至200-300纳米，但是研究人员利用这一新型的3D-SIM来捕捉多色彩、三维画面的细胞特征，他们甚至能够给细胞不同的成份标记上不同的颜色，这是一个前所未有的壮举。这一新的发展使得分子细胞生物学有了令人感兴趣的新的视角，而它又不需要任何非常规的设备，并且也并不比一台标准的商业用显微镜更难使用。（生物谷www.bioon.com）
生物谷推荐原始出处：
Science，Vol. 320. no. 5881, pp. 1332 - 1336，Lothar Schermelleh，John W. Sedat
Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy
Lothar Schermelleh,1* Peter M. Carlton,2* Sebastian Haase,2,4 Lin Shao,2 Lukman Winoto,2 Peter Kner,2 Brian Burke,3 M. Cristina Cardoso,4 David A. Agard,2 Mats G. L. Gustafsson,5 Heinrich Leonhardt,1* John W. Sedat2*
Fluorescence light microscopy allows multicolor visualization of cellular components with high specificity, but its utility has until recently been constrained by the intrinsic limit of spatial resolution. We applied three-dimensional structured illumination microscopy (3D-SIM) to circumvent this limit and to study the mammalian nucleus. By simultaneously imaging chromatin, nuclear lamina, and the nuclear pore complex (NPC), we observed several features that escape detection by conventional microscopy. We could resolve single NPCs that colocalized with channels in the lamin network and peripheral heterochromatin. We could differentially localize distinct NPC components and detect double-layered invaginations of the nuclear envelope in prophase as previously seen only by electron microscopy. Multicolor 3D-SIM opens new and facile possibilities to analyze subcellular structures beyond the diffraction limit of the emitted light.
1 Center for Integrated Protein Science, Department of Biology, Ludwig Maximilians University Munich, 82152 Planegg-Martinsried, Germany.
2 Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA.
3 Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610, USA.
4 Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany.
5 Department of Physiology and Program in Bioengineering, University of California, San Francisco, CA 94143, USA.
