几种重要植物激素的受体最近几年已被发现,包括植物生长素(茁长素)、赤霉素和脱落酸的受体,而且结构-功能研究也显示了它们的作用机制。现在,植物细胞识别“茉莉素植物激素”(生长调控、发育和防卫响应中的关键成分)也已被发现。
茉莉素受体是一个三分子复合物,由F-box 蛋白 COI1、一个JAZ (JASMONATE ZIM-domain)转录抑制因子和“肌醇戊基磷酸盐”(inositol pentakisphosphate)组成。所有三个成分都是高亲和性激素结合所必需的。用于茉莉素识别的这个体系涉及与迄今所研究的其他植物激素的机制截然不同的机制,尽管它们都取决于由激素调控的蛋白相互作用。(生物谷Bioon.com)
生物谷推荐英文摘要:
Nature doi:10.1038/nature09430
Jasmonate perception by inositol-phosphate-potentiated COI1–JAZ co-receptor
Laura B. Sheard,Xu Tan,Haibin Mao,John Withers,Gili Ben-Nissan,Thomas R. Hinds,Yuichi Kobayashi,Fong-Fu Hsu,Michal Sharon,John Browse,Sheng Yang He,Josep Rizo,Gregg A. Howe& Ning Zheng
Jasmonates are a family of plant hormones that regulate plant growth, development and responses to stress. The F-box protein CORONATINE INSENSITIVE 1 (COI1) mediates jasmonate signalling by promoting hormone-dependent ubiquitylation and degradation of transcriptional repressor JAZ proteins. Despite its importance, the mechanism of jasmonate perception remains unclear. Here we present structural and pharmacological data to show that the true Arabidopsis jasmonate receptor is a complex of both COI1 and JAZ. COI1 contains an open pocket that recognizes the bioactive hormone (3R,7S)-jasmonoyl-l-isoleucine (JA-Ile) with high specificity. High-affinity hormone binding requires a bipartite JAZ degron sequence consisting of a conserved α-helix for COI1 docking and a loop region to trap the hormone in its binding pocket. In addition, we identify a third critical component of the jasmonate co-receptor complex, inositol pentakisphosphate, which interacts with both COI1 and JAZ adjacent to the ligand. Our results unravel the mechanism of jasmonate perception and highlight the ability of F-box proteins to evolve as multi-component signalling hubs.