生物谷报道：本周Science报道细胞分裂中，中心粒凝聚的机理，发现Sgo1, Chl4和Iml3这三个基因在其中起取关键性作用，这一机理被揭示，加深了对细胞分裂机理的理解，为研究新型药物控制细胞周期过程提供理论依据。
A Genome-Wide Screen Identifies Genes Required for Centromeric Cohesion
Science,2004,29 Jan.
Adele L. Marston 1, Wai-Hong Tham 1, Hiral Shah 1, Angelika Amon 1*
1 Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17-233, 40 Ames Street, Cambridge, MA 02139, USA.
* To whom correspondence should be addressed. E-mail: angelika@mit.edu.
During meiosis, two chromosome segregation phases follow a single round of DNA replication. We identified factors required to establish this specialized cell cycle by examining meiotic chromosome segregation in a collection of yeast strains lacking all non-essential genes. This analysis revealed Sgo1, Chl4 and Iml3 to be important for retaining centromeric cohesin until the onset of anaphase II. Consistent with this role, Sgo1 localizes to centromeric regions but dissociates at the onset of anaphase II. The screen described here provides a comprehensive analysis of the genes required for the meiotic cell cycle and identified three factors important for the stepwise loss of sister-chromatid cohesion.
