Cloning and Characterization of TOM7-like Gene in Wheat
张政值，马正强
南京农业大学作物遗传与种质创新国家重点实验室，南京 210095
Abstract
通过RT-PCR技术分离了编码小麦线粒体蛋白转运酶TOM7亚基（Translocase of Outer Mitochondrial Membrane subunit 7）的cDNA，并克隆了该基因编码区的基因组DNA，将该基因命名为TaTOM7。TaTOM7是一个没有内含子的基因，其编码的多肽具有一个位于中部的疏水跨膜区和两个分别位于N-端和C-端的亲水区域。来源于真菌、动物和植物的TOM7类似蛋白在疏水跨膜区高度保守，而在亲水区变异很大。在系统发育树中，TOM7类似蛋白可以按动物、植物和真菌分为3个类群。小麦TaTOM7在基因组中以寡拷贝形式存在，它的表达在Ms2近等基因系之间和不同组织之间存在差异。中国春缺体－四体及端二体系分析表明，TaTOM7位于小麦第3染色体同源群。
Abstract
The cDNA encoding TOM7 (Translocase of Outer Mitochondrial Membrane subunit 7) like protein in wheat was cloned through RT-PCR, and its genomic DNA fragment was subsequently cloned. This gene was tentatively designated as TaTOM7. It has no intron in the coding region and its product possesses one hydrophobic trans-membrane domain in the middle, and one hydrophilic domain in the N-terminal and C-terminal domain, respectively. The amino acid composition in the trans-membrane domain of the TaTOM7 subunit is highly conserved among plants, animals and fungi. According to the phylogenetic tree, TOM7-like proteins from different species can be classified into three groups, representing plants, animals and fungi respectively. TaTOM7 is a single- or oligo-copy gene in the wheat genome, displaying different expression levels between Ms2 near-isogenic lines in some tissues, suggesting a role of Ms2 in its expression. TaTOM7 was mapped to chromosome group three.
该文发表在：遗传学报 2005.2 Vol 32, No.2 170-177
