先将目标RNA分子插入到天然的tRNA支架上，这种杂合的RNA既可以避免RNase的酶水解，又可以高保真地复制出大量的RNA分子。
生物谷报道：一项在体内表达功能性RNA分子的方法，为简化RNA样品的准备打开了一扇方便之门，也为体外、体内研究RNA结构、相互作用和功能提供了一套有用工具。
RNA新地位的发现，大大增加了RNA研究的重要性。进一步加深对不同种类RNA的理解，甚至将之开发成为潜在的药物靶点，都需要在体内、体外综合研究RNA的结构和功能。但是，在这点上恰恰是当前研究所欠缺的，部分原因在于缺乏合适的RNA实验材料。一项新的方法——运用tRNA支架（tRNA scaffold），可在体内大量表达RNA分子，为解决此类问题提供了一条非常有借鉴价值的解决方案。
部分英文原文：
Nature Methods - 4, 571 - 576 (2007)
Published online: 10 June 2007; | doi:10.1038/nmeth1058
Recombinant RNA technology: the tRNA scaffold
Luc Ponchon & Frédéric Dardel
Cristallographie & RMN Biologiques, Université Paris Descartes, CNRS, 4 avenue de l'Observatoire, 75006, Paris, France.
Correspondence should be addressed to Frédéric Dardel frederic.dardel@univ-paris5.fr
RNA has emerged as a major player in most cellular processes. Understanding these processes at the molecular level requires homogeneous RNA samples for structural, biochemical and pharmacological studies. So far, this has been a bottleneck, as the only methods for producing such pure RNA have been in vitro syntheses. Here we describe a generic approach for expressing and purifying structured RNA in Escherichia coli, using tools that parallel those available for recombinant proteins. Our system is based on a camouflage strategy, the 'tRNA scaffold', in which the recombinant RNA is disguised as a natural RNA and thus hijacks the host machinery, escaping cellular RNases. This opens the way to large-scale structural and molecular investigations of RNA function.
