生物谷：研究人员用一种新技术来分析基因和蛋白质的表达来获得对衰老以及糖尿病的更详细的信息。以前，科学家曾了解带有daf-2基因突变的美丽线虫比没有突变的线虫的寿命长一倍。daf-2降低一个名为“类胰岛素信号发生”过程的活性，从而延长了寿命。Meng-Qui Dong和同事用质谱仪比较了带有daf-2突变的线虫和不带突变的线虫的蛋白质的量。他们识别出47个蛋白质在突变线虫中更丰富，另有39个蛋白质在突变线虫中的量不丰富。实验显示，有些蛋白质增加寿命、有些降低寿命。Stuart K. Kim在一篇相关的研究评述中写道：研究人员将继续改进这个方法，因为这个技术现在只能用来研究线虫基因组中不到10%的蛋白质。
原始出处：
出处一：
Science 3 August 2007:
Vol. 317. no. 5838, pp. 660 - 663
DOI: 10.1126/science.1139952
Quantitative Mass Spectrometry Identifies Insulin Signaling Targets in C. elegans
Meng-Qiu Dong,1 John D. Venable,1 Nora Au,2,3 Tao Xu,1 Sung Kyu Park,1 Daniel Cociorva,1 Jeffrey R. Johnson,1 Andrew Dillin,2 John R. Yates, III1*
DAF-2, an insulin receptor–like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.
1 Scripps Research Institute, La Jolla, CA92037, USA.
2 Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
3 University of California at San Diego, La Jolla, CA 92093, USA.
* To whom correspondence should be addressed. E-mail: jyates@scripps.edu
出处一：
Science 3 August 2007:
Vol. 317. no. 5838, pp. 603 - 604
DOI: 10.1126/science.1146805
CELL BIOLOGY:
Proteins That Promote Long Life
Stuart K. Kim
Quantitative analysis of proteins in a long-lived worm reveals changes that both increase and shorten life span
