近日，美国美国梅奥临床医学院的研究人员发现，钠氢交换调控因子(NHERF-2)能够维持内皮细胞的稳定。相关研究成果于5月17日发表在Blood上。
钠氢交换调控因子(NHERF-2)是几乎所有内皮细胞(ECs)所含有的组分，但是到目前为止，它的功能还不明确。
这里，研究人员表示，NHERF-2是内皮细胞的一个关键性的调节因子。他们发现，在没有有丝分裂原（如VEGF）的环境下，对比于控制组ECs，NHERF-2沉默的ECs仍然能够以很快的速率进行增殖。
进一步研究表明，NHERF-2沉默EC之所以能够表现出高速增殖表型，是因为它具有加速的细胞周期，这可能是被这些因素协同引起的：细胞质钙浓度增加，的c-Myc表增加达，细胞周期蛋白D1表达增加，p27表达下降。
利用人类血管瘤的小鼠模型，他们发现，由NHERF-2沉默细胞引起的内皮细胞瘤，在个体上要大于由控制组细胞所诱导的肿瘤。
Debabrata Mukhopadhyay表示，NHERF-2是内皮增生的一个负调控因子，它对内皮的稳定及血管构建也可能具有重要作用。（生物谷Deepblue编译）
doi: 10.1182/blood-2011-11-392563
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PMID:
NHERF-2 maintains endothelial homeostasis
Resham Bhattacharya, Enfeng Wang, Shamit K. Dutta, Pawan K. Vohra, Guangqi E, Y. S. Prakash, and Debabrata Mukhopadhyay.
The Na+/H+ exchanger regulatory factor-2 (NHERF-2) is an integral component of almost all endothelial cells (ECs), yet its endothelial function is not known.Here, we found that NHERF-2, is a key regulator of endothelial homeostasis because NHERF-2–silenced ECs proliferate at a much higher rate even in the absence of mitogens such as VEGF compared with control ECs.We further show that the hyperproliferation phenotype of NHERF-2–silenced EC is because of an accelerated cell cycle that is probably caused by a combination of the following factors: increased cytoplasmic calcium, increased expression of c-Myc, increased expression of cyclin D1, and reduced expression of p27.Using an experimental mouse model of human hemangioma, we found that the endothelial neoplasms derived from NHERF-2–silenced cells were much larger in volume than those derived from control cells.Thus, NHERF-2 is a negative regulator of endothelial proliferation and may have important roles in endothelial homeostasis and vascular modeling
